|
Analysis of NASFIT powder
Carried out by Analytical Testing Laboratories.
Inborn Error of Metabolism & Drug testing services.
Beirut- Lebanon.
NASFIT:
Presented as a fine mixed powder,
dried, Brown, odorless
* Methods of
Extraction :
(1) 10 grams extracted in boiling water for 1 hour
(2) 10 grams extracted in boiled water for 1 hour
(3) 10 grams extracted in sodium Hydroxide ( Na oH) at 0.1 N, the pH
corrected to 7.0 using phosphoric acid.
(4) 10 grams extracted in sodium Hydroxide ( Na oH) at 0.1 N, the pH was 5.2
.
* Groups of
contents analyzed:
- Sugar Analysis
- Alkaloids Screen
- Sterols Screen
- Solvent Screen
- Fatty Acids Methyl Ester Profile
- Amino Acids Methyl Ester Profile
- Organic Acids
*
Methods of Analysis :
- High Performance Liquid Chromatography ( HPLC)
- Gas Chromatography / Mass Spectrometry
- Gas Chromatography
- Amino Acid Analyzer
*
Results of the boiled and boiling extract :
|
Grps.
|
*Sugar
|
Concentrations
|
Graph &
Structure
|
|
|
1- Fructose
2- Glucose
3- Sucrose
|
2.15 g/100ml
1.53 g/100ml
0.0.3 g/100ml
|
GR No. 1
|
|
|
*Alkaloids
|
Concentration
|
Graph &
Structure
|
|
|
1- Carbafuran
2- Trimethoxyamphetamine
3- Caffeine
|
|
GR No. 2
|
|
|
*Sterols
|
Concentration
|
Graph & Structure
|
|
|
1- Sterol
|
Not detected
|
GR No. 3
|
|
|
* Fatty Acids
|
Concentration
|
Graph & Structure
|
|
|
1-Palmitic (C16:0)
2- Lenoleic ( C18:2)
|
11.30%
16.65%
|
GR No.
5
|
|
|
*Amino Acids
|
Concentration
5 x Dilution
|
Graph & Structure
|
|
|
1- Glutamic acid
2- Asparagine
3- Serine
4- Glutamine
5- Glycine
6- Threonine
7- Histidine
8- Alanine
9- Arginine
10- Tyrosine
11- Valine
12- Isoleucine
13- Tryptophan
14- Phenylalanine
15- Leucine
16- Ornithine
17- Lysine
|
1129
µmoles/L
4023 µmoles/L
210 µmoles/L
1849 µmoles/L
103 µmoles/L
131 µmoles/L
125 µmoles/L
389 µmoles/L
384 µmoles/L
90 µmoles/L
281.45 µmoles/L
159.5 µmoles/L
120.8 µmoles/L
83.83 µmoles/L
139.2 µmoles/L
61.25 µmoles/L
142.3 µmoles/L
|
GR No.
6
|
|
|
* OrganicAcids
|
Concentration
|
Graph & Structure
|
|
|
1- Lactic
2- Glyoxylic
3- Pyrovic
4- Acctoacetic
5- Succinic
6- Malic
7- Pyroglutamic
8- Citric
9- Peak # 72
|
------------------------------------------
---------------------
---------------------
---------------------
---------------------
---------------------
20.19 %
|
See Picture
|
Very Long Chain
Fatty Acids in Blood
NASFIT POWDER ( NASFIT EXTRACTED POWDER)
Pasteur Cerba - Paris
|
|
Concentration
(Normal Control)
|
Concentration
(HIV Positive)
|
Ref. µmoles/L
|
|
Test
|
T1
|
T2
|
T3
|
T1
|
T2
|
T3
|
|
|
C22:0
|
29.20
|
31.50
|
43.70
|
34.20
|
33.30
|
40.20
|
49.90
|
|
C23:0
|
12.40
|
14.80
|
19.80
|
13.50
|
12.30
|
17.70
|
22.20
|
|
C24:0
|
30.30
|
33.40
|
47.50
|
19.40
|
17.30
|
22.50
|
40.90
|
|
C24:0
|
28.10
|
29.10
|
40.60
|
28.80
|
26.10
|
36.50
|
37.60
|
|
C25:0
|
0.86
|
0.92
|
1.22
|
0.76
|
0.61
|
1.10
|
1.41
|
|
C26:0
|
0.58
|
0.62
|
0.70
|
0.38
|
0.48
|
0.68
|
0.68
|
|
Pristanic A.
|
0.66
|
0.76
|
0.61
|
0.52
|
0.51
|
0.35
|
0.70--0.57
|
|
Phytanic A.
|
0.64
|
1.18
|
1.30
|
1.25
|
1.20
|
1.50
|
2.60
|
(T1)
Zero
Time
American University Of Science
(T2) After (1) Hour
and Technology
(T3) After ( 24) Hours
(A U S T)
NON ESTERIFIED FATTY ACIDS:
Colorimetry
0 Time 1120 µmole/L
1 Time 1120 µmole/L
2 Time 1281 µmole/L
3 Time 823 µmole/L
(0) Zero Time
(1) After (1) Hour
(2) After (2) Hours
(3) After 24 Hours
Dose : (0.8 ml / kg body weight) of total solution every 8 Hrs.
Pasteur Cerba
Results of Nasfit Basic ( Na oH)
at pH 7.0)
Method file name : AMERICA
. MET
Mass Spectrum CHROMATOGRAPHY
Boiling
|
AMINO ACIDS
|
Concentration
|
|
1- Glutamic Acid
|
304.1 µmoles/L
|
|
2- Asparagine
|
3312.5 µmoles/L
|
|
3- serine
|
159.4 µmoles/L
|
|
4- Glutamine
|
67.3 µmoles/L
|
|
5- Glycine
|
140.9 µmoles/L
|
|
6- Threonine
|
87.3 µmoles/L
|
|
7- Histidine
|
87 µmoles/L
|
|
8- Citrulline
|
30.9 µmoles/L
|
|
9- Alanine
|
278.6 µmoles/L
|
|
10- Arginine
|
259.8 µmoles/L
|
|
11- Taurine
|
101.9 µmoles/L
|
|
12 – Tyrosine
|
119.5 µmoles/L
|
|
13- Valine
|
243.7 µmoles/L
|
| 
